Fig. 3

miRNA expression in cultured cells and supernatants. (a) 2D ddPCR plots of miR-222-3p in the three indicated cell lines (upper) and their culture supernatants (lower). All cDNAs from cells were diluted 1:100 for accurate Poisson distribution analysis. (b) miR-141-3p, miR-221-3p and miR-222-3p were quantitated in cells (dots) and supernatants (diamonds). All data are normalized for total mRNA content, and expressed as copy/μl of miRNA in the original samples. Standard deviation was calculated from three independent experiments