Fig. 1

Effect of cisplatin on proliferation and ER-α36 expression of breast cancer cells. a MCF-7 and MCF-7/DDP cells were treated with increasing concentrations of cisplatin (DDP) for 48 h, and then cell proliferation was measured using CCK-8 assay kit. b Cisplatin sensitivity of MCF-7 and MCF-7/DDP cells was examined by monolayer colony formation assay. c ER-α36 protein expression in MCF-7 and MCF-7/DDP cells was analyzed using western blot. d The quantitative analysis of ER-α36 expression of (c). e MCF-7 cells were treated with or without 5 μg/mL cisplatin for 48 h, cisplatin-induced expression of ER-α36 was measured by western blot. f The quantitative analysis of cisplatin-induced ER-α36 expression of (e). g MCF-7, BT474 and MDA-MB-231 cells were treated with cisplatin at the indicated concentrations for 48 h and then the protein levels of ER-α36 were analyzed by western blot. h The quantitative analysis of cisplatin-induced ER-α36 expression of (g). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001