Fig. 4
The influence of the chemoimmunotherapy on infiltration and activity stage of lymphocyte subpopulations infiltrating MC38 tumors. The figure presents scheme of multiparameter flow cytometric analysis of CTLs, Th, Treg, NK, NKT and B cells (a), as well as changes in their proportions after therapy (b). The effector cell subpopulations were distinguished on the base of CD44 and CD62L expression (c). To calculate the mean ± SD at least 5 mice per group were analyzed. The differences between the groups were estimated using nonparametric Kruskal-Wallis test followed by Dunn’s multi comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001)