Fig. 6

UFC1 promotes gastric cancer growth through the regulation of Lin28b. a RT-PCR analyses of Lin28b expression in control and UFC1 knockdown GC cells. b GC cells were co-transfected with Lin28b 3’-UTR reporter plasmid wild type or mutant with sh-UFC1 or UFC1 plasmid. The luciferase activity was determined by using dual-luciferase reporter assay. c The association between Lin28b and UFC1 expression levels was determined by using Pearson correlation analysis. d GC cells were transfected with sh-UFC1 in the presence or absence of Lin28b. The proliferating ability of GC cells was determined by using cell colony formation assay. e Cell cycle distribution was determined by using flow cytometry. f Cell apoptosis was determined by using flow cytometry. g The migration ability of GC cells were determined by using transwell migration assay. h The invasion ability of GC cells were determined by using matrigel invasion assay. i Tumor growth curves and tumor weights for mice in sh-control, sh-UFC1, and sh-UFC1 + Lin28b groups. j HE staining and immunohistochemical staining of Ki-67 for tumor tissues from mice in sh-control, sh-UFC1, and sh-UFC1 + Lin28b groups. Scale bar: 100 μm