Fig. 5

SPOP suppresses cell proliferation, invasion and migration partially dependent on ATF2. a Cell proliferation assay (left panel) and western blot (right panel) of C4–2 cells infected with lentivirus expressing the indicated shRNAs. All data shown are mean values ± SD (error bar) from three independent experiments. *p < 0.05 in C4–2 cells at day 7. b Assays shown in (A) were performed in C4–2 cells infected with empty vector or lentivirus expressing HA-SPOP-F133 V in combination with control shRNA or ATF2-spefic shRNAs. c and d Cell migration (C) and invasion (D) assay of C4–2 cells infected with lentivirus expressing the indicated shRNAs. All data shown are mean values ± SD from three replicates. *p < 0.05. e Cell migration and invasion f assay of C4–2 cells infected with lentivirus expressing HA-SPOP-F133 V in combination with control shRNA or ATF2-spefic shRNAs. All data shown are mean values ± SD from three replicates. *p < 0.05. g Quantitative RT-qPCR measurement of mRNA expression of three ATF2 transcriptional targets SOX9, MMP9, and TGFB2 in C4–2 cells. GAPDH mRNA levels were used for normalization. Standard deviation (S.D.) of at least three independent experiments is shown to indicate statistical significance. *p<0.05,**p<0.01, **p<0.001