Fig. 1

CPNE1 overexpression in NSCLC. a Data on CPNE1 mRNA expression in lung adenocarcinoma, squamous cell carcinoma and normal lung tissue from several study groups deposited in the Oncomine database (http://www.oncomine.org). b CPNE1 mRNA levels in 60 NSCLC tissues and paired noncancerous lung tissues. c Formalin-fixed and paraffin-embedded NSCLC tissues (n = 20) were subjected to IHC analyses of the CPNE1 protein. Representative images of CPNE1 antibody staining (high, moderate and low) in NSCLC tissues and normal tissues. d Effect of the CPNE1 expression level on overall survival in 1926 lung cancer patients; the Kaplan-Meier plots were generated using a Kaplan-Meier Plotter (http://www.kmplot.com). e The level of CPNE1 in human NSCLC cells was detected by qRT-PCR and western blot analysis. *P < 0.05; **P < 0.01; ***P < 0.001