Fig. 6

Effects of okadaic acid (OA) on JS-K-induced cell apoptosis and PP2A activation in SMMC7721 and HepG2 cells. a Effect of OA treatment as PP2A inhibitor on cell apoptosis. The cells were pretreated with OA (1 nM) for 1 h, and then stimulated with JS-K (10 μM) for 24 h. The apoptosis was assessed by flow cytometry. b Effect of OA treatment as PP2A inhibitor on the expression of apoptotic-related protein. c Effect of OA treatment as PP2A inhibitor on the expression of PP2A-C and its substrates. The cells were pretreated with OA (1 nM) for 1 h, and then stimulated with JS-K (10 μM) for 24 h. The expressions of protein were assessed by Western blotting analysis. Data are mean ± SD. n = 3 for each concentration. *P < 0.05,**P < 0.01, vs. control group, ^#P < 0.05, ^##P < 0.01 vs. cells treated with JS-K alone