Fig. 6

miR-182 suppresses invadopodia formation of lung cancer cells via suppression of the Cdc42/N-WASP pathway. a A549 and H1299 cells were pretreated with 500 mM of PDBu for 0 min, 15 min, 30 min, 60 min and 120 min, and then N-WASP protein expression were measured by Western blotting. b A549 and H1299 cells were transfected with scrambled miRNA, miR-182, si-NC or CTTN siRNA with or without PDBu or HGF treatment, then subjected to analysis of CTTN, CDC42 and N-WASP expression by Western blotting. c Arp2 expression in A549 cells treated with or without HGF or PDBu was detected by immunoblotting. β-actin was used as a control. d and e A549 and H1299 cells were transfected with scrambled miRNA, miR-182, si-NC, CTTN siRNA, or N-WASP siRNA was co-transfected with miR-182 in the presence of PDBu treatment for 30 min, and stained for cortactin(green), F-actin (red), and nuclei (blue). Cells with invadopodia were quantified. Representative images are displayed on the right. Data are shown as the mean ± SD. Scale bar, 50 μm, 40× magnification, *P < 0.05, **P < 0.01. f A549 and H1299 cells were transfected with si-NC, CTTN siRNA or Cdc42 siRNA, then cortactin, Cdc42, N-WASP and Arp2 protein expression were measured by Western blotting. β-actin was used as a control. g Following treatment with 0,10, and 20 μM PP2, A549 and H1299 cells were harvested. Levels of Cdc42, and N-WASP, and the phosphorylation of cortactin were determined using Western blot analysis. β-actin was used as a control