Fig. 1

Expression of RAP1A in CRC and matched adjacent normal tissues. a: qRT-PCR assay indicates the mRNA level of RAP1A gene was higher in CRC tissues than that in matched adjacent normal tissues (n = 23, p = 0.0015). b: Western blot indicates the protein level of RAP1A was higher in CRC tissues than that in matched adjacent normal tissues (n = 23, p < 0.0001). c: Representative protein bands of RAP1A and β-actin. d: Representative immunohistochemical staining images of RAP1A in CRC and matched adjacent normal tissues. e: The cut-off value of immunohistochemical staining scores was determined by receiver operating characteristic curve analysis, with sensitivity of 70.7% and specificity of 57.3%. ** means p < 0.01