Fig. 3

MNX1 overexpression accelerates bladder cancer cell proliferation. a MNX1 was stably overexpressed and silenced in T24 and 5637 cells. MNX1 expression was verified by western blotting. α-Tubulin was used as the loading control. b MTT assay assessment of cell viability. c and d Representative images (left) and quantification (right) of colony formation assay. Bars represent the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01. shRNA, short hairpin RNA