Fig. 1

PAX8 inhibits the migration and invasion of gastric cancer cells in vitro. a Real-time PCR analysis of PAX8 mRNA levels in 19 pairs of gastric cancer and adjacent noncancerous tissues. b Analysis of PAX8 protein (upper) and mRNA (lower) expression in indicated cell lines by real-time PCR and Western blotting, respectively. Numbers below Western blots indicate fold change relative to the value in GES-1 cells. ^*P < 0.05 vs. GES-1 cells. c Western blot analysis of PAX8 protein levels in AGS and SGC-7901 cells transfected with PAX8-expressing plasmid or empty vector. d Measurement of the proliferation of AGS and SGC-7901 cells transfected with PAX8-expressing plasmid or empty vector after culturing for 48 and 72 h. N.S. indicates no significance. e In vitro wound-healing assay was performed to assess the migrative capacity of AGS and SGC-7901 cells transfected with PAX8-expressing plasmid or empty vector. ^*P < 0.05 vs. vector-transfected cells. f Transwell invasion assay was used to determine the invasive ability of AGS and SGC-7901 cells transfected with PAX8-expressing plasmid or empty vector. ^*P < 0.05 vs. vector-transfected cells