Fig. 7

PAX8 overexpression suppressed tumor angiogenesis and metastasis in nude mice. SGC-7901 cells stably expressing PAX8 or empty vector were injected through the tail vein, and lung metastasis was assessed. a Representative photographs of the lung with metastatic lesions (arrowhead). b Representative H&E-stained lung tissue sections. Scale bar = 50 μm. c The number of metastatic lesions in the lung was determined (n = 6). d Analysis of tumor angiogenesis by CD31 immunostaining. PAX8 overexpression caused a reduction in CD31-positive MVD (n = 6). Scale bar = 80 μm. e Real-time PCR analysis of miR-612 levels in the lung tissue (n = 6). f Western blot analysis of FOXM1 and PAX8 protein levels in the lung tissue (n = 6). Top, representative blots from 1 to 3 mice are shown