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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Osteopontin alters DNA methylation through up-regulating DNMT1 and sensitizes CD133+/CD44+ cancer stem cells to 5 azacytidine in hepatocellular carcinoma

Fig. 1

OPN regulates the properties of CD133+/CD44+ cells. a OPN was tightly correlated with the expression of CD133 and CD44 in the clinical samples (Data from TCGA). b The expression level of OPN during generation passaging. c OPN knockdown in CD133+/CD44+ cells decreased the number of sphere formation significantly, 100×, **, p < 0.01. d Knockdown of OPN impeded the migration on gelatin of CD133+/CD44+ cells. e Stemness-related genes were down-regulated by comparing with CD133+/CD44+ SCR and CD133+/CD44+ shOPN. f In vivo, 5000 cells of CD133+/CD44+ SCR and CD133+/CD44+ shOPN were subcutaneously implanted, shOPN delayed the emergency and slowed down the increase rate of tumor, *, p < 0.05 and ***, p < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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