Fig. 2

CETSA-based determination of binding between oxaliplatin and tNOX. A, B, CETSA curves of tNOX in p53-wild-type cells (a) and p53-null cells (b) were determined in the absence and presence of 100 μM oxaliplatin, and cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-Actin was used as an internal control. Representative images are shown. The band intensities of tNOX were normalized with respect to the intensity at 40 °C. The denaturation midpoints were determined using a standard process