Fig. 7

Oxaliplatin induces intracellular ROS generation, resulting in p53 phosphorylation, POU3F2 up-regulation, and apoptosis in p53-wild-type cells, but not in p53-null cells. a, The percent change in intracellular ROS generation was measured after cells were exposed to oxaliplatin for 1 h. b, Cells were treated with oxaliplatin for 24 h, and cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-Actin was used as an internal control. Representative images are shown. c, Cells were exposed to oxaliplatin and the RNA levels of POU3F2 were analyzed by RT-PCR. d, e, Cells were pre-incubated with or without 10 mM NAC at 37 °C for 2 h before exposure to oxaliplatin. The percentage of apoptotic cells was also determined by flow cytometry; the presented values (mean ± SEs) represent at least three independent experiments (**p < 0.01, ***p < 0.001 for treatments vs. controls) (d). Cell lysates were separated by SDS-PAGE and analyzed by Western blotting. β-Actin was used as an internal control. Representative images are shown (e)