Fig. 2

PBX3 is required for the induction of mesenchymal transition and cell migration and invasion in response to TGF-β. a qRT-PCR analysis of PBX3 expression in response to TGF-β (5 ng/mL) induction in U87 and U251 cells at different time points, as indicated. b Western blot analysis of PBX3 expression in response to TGF-β (5 ng/mL) induction in U87 and U251 cells at different time points, as indicated. c U87 and U251 cells stably expressing LV-siRNA-PBX3 or LV-siRNA-NC were treated with TGF-β for 48 h and immunoblotting analysis of PBX3, N-cadherin, ZEB1, Slug and CD44 were performed. d and e Quantification of wound-healing (d) and transwell assays (e). **indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + TGF-β (−) group and LV-siRNA-PBX3 + TGF-β (−) group. $ indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + TGF-β (−) group and LV-siRNA-NC + TGF-β (+) group. # indicates a statistical significant difference (p < 0.01) between LV-siRNA-NC + TGF-β (+) group and LV-siRNA-PBX3 + TGF-β (+) group