Fig. 1

Experimental design and workflow for quantitative proteomics analysis of Ctrl-K562 and Curcu-K562 cells. The SWATH-MS analysis was performed using a Triple TOF 5600+ system equipped with a Nanospray III interface and coupled to an Eksigent nanoLC 425. For quantitative analysis, 1 μg of each sample was subjected to SWATH acquisition (34 swaths of 25 Da); two mass spectrometric technical replicates were acquired for each of the two biological replicates. To generate the spectral reference library, 2 μg of the pooled sample were subjected to traditional data-dependent acquisition (DDA). A library of 1967 proteins was created. After performing the extraction and integration processes using PeakView Software v2.2, 1796 proteins were exported into MarkerView 1.2.1 that together with GraphPad7.0 and Microsoft Excel 2010, was used for statistical analysis. BR: Biological Replicate; TR: Technical Replicate