Fig. 1
LncRNA PART1 is upregulated in gefitinib-resistant ESCC cells. a Western blot analysis of EGFR expression levels in five ESCC cell lines. b CCK8 assay was performed to evaluate the cell viability of five cell lines after treatment with indicated concentration of gefitinib for 48 h. c Presentation of the established gefitinib-resistant cell lines with specific morphologic changes. d, The IC50 value of gefitinib was detected for both sensitive and resistant cells by cell viability assay. *P < 0.05 compared to ESCC parental cell group. e, The cell viability of both gefitinib-resistant and sensitive cells were also detected. *P < 0.05 compared to ESCC parental cell group. f, LncRNA microarray data of two-paired gefitinib-resistant and parental cells are presented in a heatmap. g, Determination of IC50 values of gefitinib for both resistant cell lines after transfection with various siRNAs. ***P < 0.001 compared to Ctrl siRNA group