Fig. 7

WIPF1 expression antagonizes the tumor suppressive effect of miR-141/200c and correlates with poor patient survival. a WIPF1 with the deletion of its 3’-UTR (WIPF1/− 3’-UTR) blocked the inhibitory effect of miR-141/200c on cell migration and invasion of PDAC. PANC-1 cells were infected with either an empty vector as control or lentivirus containing WIPF1 cDNA that encodes the entire coding sequence minus its 3’-UTR, together with lentivirus containing either miR-141 or miR-200c. Data represent the mean ± SD with three independent experiments. ^** P < 0.01. b-d Expression of WIPF1/− 3’-UTR blocked the inhibitory effect of miR-141/200c on tumor metastasis. PANC-1 cells were infected as described in (a), and the resulting cells were injected into the spleen of NOD/SCID mice. Representative livers and lungs from the animals are shown. H&E staining was performed. Metastatic tumour nodules (T) outlined by a dotted line. Magnification, × 100. Student’s t-test was used to analyze the difference between the two groups. e High expression of WIPF1 in PDAC correlates with poor patient survival. Data was derived from 177 patients in the TCGA database. Kaplan-Meier method was used to display the survival curve. Statistical analysis was performed using the log rank test