Fig. 1

ANXA2 promotes ESCC cell motility and invasion in vitro and lung metastasis in mice. a-b Western blot analysis of ANXA2 expression in ESCC cell lines (a) or ESCC cells transfected with the indicated siRNA or plasmid construct (b). c-e ESCC cells were transfected with the indicated siRNA, shRNA or plasmid construct. c Cell motility was assessed by the wound-healing assay. d-e Cell migration and invasion abilities were examined using transwell assays. Representative results (left) and statistical plots (right) are shown. f Cell metastatic potential was evaluated using an in vivo pulmonary metastasis assay. Representative photos of fixed lung tissues (left top) and the results of H&E staining (left bottom) are shown. The arrows indicate the lung metastatic nodules. The number of metastatic nodules was plotted (right). **, P < 0.01, ***, P < 0.001