Fig. 4

Phosphorylation at Tyr23 mediates nuclear localization of ANXA2. a Interaction between ANXA2 and MYC in ESCC cells was detected with an immunoprecipitation-Western blot assay. b Cellular localization of exogenously expressed ANXA2-Y23D or ANXA2-Y23A (green) was detected by immunofluorescence staining using the anti-His antibody. DAPI was used to stain nuclei (blue). Scale bar = 30 μM. c ESCC cells were transfected with the indicated constructs, and the nuclear and cytoplasmic levels of exogenously expressed ANXA2-Y23D or ANXA2-Y23A were assessed via immunoblotting. d Western blot analysis of p-ANXA2 expression in ESCC cell lines. e Cell migration and invasion abilities examined with transwell assays. Representative results (left) and statistical plots (right) are shown. *, P < 0.05; **, P < 0.01