Fig. 3

RIF1 enhances cancer stem cell-like properties in vitro. a GSEA plot showing RIF1 expression level was positively correlated with activated stemness-related gene signatures in the GEO data set. (NCBI/GEO/GSE7463; n = 43). b and c Representative images of spheres formed by the OVCAR3 and A2780 cells. Scale bar, 100 μm. d and e Quantification of sphere-formation efficiency of the indicated cells. f Western blot analysis of the protein expression of the stemness-associated markers including NANOG and SOX2 in RIF1-silenced and overexpressed OVCAR3 and A2780 cells. Date were shown as mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01