Fig. 6

Knockdown of hTERT abrogated the promotion of cell viability, colony formation and sphere formation mediated by RIF1 overexpression in EOC cells. a Efficiency of hTERT knockdown (hTERT KD) in OVCAR3 and A2780 cells by shRNA was verified by real-time qPCR and western blot. b Cell viability at 72 h was examined by absorbance at the wavelength of 490 nm and normalized to the absorbance value in the control group by MTS assay. c and d Colony formation assay of control vector or RIF1-overexpressed OVCAR3 and A2780 cells with or without hTERT knockdown. e and f Representative images and quantification of spheres formed by control vector or RIF1-overexpressed OVCAR3 and A2780 cells with or without hTERT knockdown. Scale bar, 100 μm. The results were shown as means ± SD of three independent experiments. * P < 0.05, ** P < 0.01