Fig. 8
Knockdown of RIF1 inhibits tumourigenicity of EOC cells in vivo. a and b Efficiency of RIF1 stable knockdown (RIF1 KD) in OVCAR3 cells by lentivirus was verified by real-time qPCR (a) and western blot (b) after 30 generations of cell culture. c and d. 2 × 106 RIF1 stable-knockdown and vector control OVCAR3 cells were inoculated subcutaneously into the right and left flanks of nude mice. Tumor weight and volume of xenografts were evaluated. e Representative images of H&E and IHC staining of the resected tumor. f and g IHC analysis showed that RIF1 knockdown reduced the expression of hTERT and decreased the proliferation index Ki67. Data were presented as means ± SD. * P < 0.05, ** P < 0.01