Fig. 2

Similar cell proliferation and hypoxia, but differential ductal breakthrough in primary tumors of 4T1 + RAW264.7 versus 4T1 intraductally inoculated mice. Immunohistochemistry for the cell proliferation marker Ki67, hypoxia-associated enzyme carbonic anhydrase IX (CAIX) and myoepithelial cell marker cytokeratin 5 was performed on paraffin sections of primary tumors at 1, 3 and 5 w p.i. from 4T1 + RAW264.7 and 4T1 inoculated mice. The percentage of Ki67-, CAIX- and cytokeratin 5-positive staining was quantified (n = 5 at each time point and for each inoculation group). Scale bars = 50 μm. All data are presented as the means +/− SEM. NS: not significant, ***: P < 0.001