Fig. 6

Immunohistochemistry of the tumor immune microenvironment in 4T1 + RAW264.7 versus 4T1 intraductally inoculated mice. Immunohistochemistry for immune cell markers CD45 (pan-immune cell marker), CD163 (anti-inflammatory macrophage marker), Ly6G (neutrophil marker) and CD8a (cytotoxic T-cell marker) was performed on paraffin sections of primary tumors at 1, 3 and 5 w p.i. from 4T1 + RAW264.7 and 4T1 inoculated mice. The percentage of CD45-, CD163-, Ly6G- and CD8a-positive staining was quantified (n = 5 at each time point and for each inoculation group). Dashed inserts and arrowheads highlight the few Ly6G- and CD8a-positive cells at 1 w p.i. Black scale bars = 50 μm; red scale bars = 20 μm. All data are presented as the means +/− SEM. NS: not significant, *: P < 0.05