Fig. 8

CHI3L1 and LCN2 as immune-associated disease biomarkers in 4T1 + RAW264.7, 4T1 and RAW264.7 intraductally inoculated mice. a, b CHI3L1 (a) and LCN2 (b) levels secreted in cell-free culture media of 24 h mono- and co-cultures of 4T1 mammary tumor cells and RAW264.7 macrophages (n = 3 for all culture conditions). c, d Primary tumor/mammary gland CHI3L1 (c) and LCN2 levels (d) at 3 and 5 w p.i. in 4T1 + RAW264.7, 4T1 and RAW264.7 inoculated mice (4T1 + RAW264.7 inoculation group: n = 8 tumors at 3 w p.i. and n = 12 tumors at 5 w p.i.; 4T1 inoculation group: n = 8 tumors at 3 w p.i. and n = 6 tumors at 5 w p.i.; RAW264.7 inoculation group: n = 5 mammary glands at 3 and 5 w p.i.). e, f Serum CHI3L1 (e) and LCN2 levels (f) at 3 and 5 w p.i. in 4T1 + RAW264.7, 4T1 and RAW264.7 inoculated mice (4T1 + RAW264.7 inoculation group: n = 6 sera at 3 w p.i. and n = 7 sera at 5 w p.i.; 4T1 inoculation group: n = 6 sera at 3 and 5 w p.i.; RAW264.7 inoculation group: n = 4 sera at 3 and 5 w p.i.). g, h Splenic CHI3L1 (g) and LCN2 levels (h) at 3 and 5 w p.i. in 4T1 + RAW264.7, 4T1 and RAW264.7 inoculated mice (n = 5 at each time point and for each inoculation group). All data are presented as the means +/− SEM. NS: not significant, *: P < 0.05, **: P < 0.01, ***: P < 0.001