Fig. 7
DLBCL exosomes promote cell proliferation, migration and angiogenesis in vitro. a Proliferation of stromal cells after 96 h of incubation with increasing concentrations of OCI-LY3 EXOs were evaluated by an MTT assay. Data are representative of three independent experiments. (two-tailed t test, *P < 0.05). b DLBCL cells were co-incubated with supernatants of BMSCs that were treated with or without increasing concentrations of OCI-LY3 EXOs for 24 h. After 5 days incubation, cell viability was assessed by an CCK8 assay. Data are reported as the percentage relative to control (n = 3). *P < 0.05; **P < 0.01. c (Left) Microscopy images of wound healing assay showing closure of the scratch when HUVECs were cultured in presence or absence of OCI-LY3 EXOs (50 or 100 μg/mL) for 24 h. Scale bar = 10 μm. (Right) Wound closure (μm2) was quantified using ImageJ software. (n = 3). d (Left) HUVECs were exposed to 50 or 100 μg/mL OCI-LY3 EXOs (50 and 100 μg/mL) or PBS (Control) for 30 min, and then seeded on Matrigel for 3 h. Scale bar = 10 μm. Quantification of several parameters of the tube formation assay using Image J (n = 5). (a) Nodes; (b) Tubes; (c) Branching points; (d) Tubes length. *P < 0.05, **P < 0.01. e Matrigel plug assay performed by subcutaneous injection of Matrigel mixed with or without OCI-LY3 EXOs (100 μg/ml) in 3 mice. Representative of H & E staining of matrigel plug: (a) PBS (200 ×); (b) OCI-LY3 EXOs (200 ×); (c) Quantitation of the total number of cells within the matrigel plug; (d) OCI-LY3 EXOs (400 ×). Representative of immunofluorescence of CD31 and DAPI co-staining of the matrigel plug: (e) PBS (200 ×; (f) OCI-LY3 EXOs (200 ×); (g) OCI-LY3 EXOs (H & E staining, 400 ×); (h) OCI-LY3 EXO (400 ×). f PBS (control) or 100 μg OCI-LY3 EXOs were mixed with OCI-LY3 cells and subcutaneously injected into the NOD/SCID mice, and then xenograft tumors were monitored. (N = 3) *P < 0.05. g Representative images of subcutaneous tumors removed from NOD/SCID mice at 56 days