Fig. 5
Effect of LGR5 on intracranial tumor growth and overall survival time of xenograft mice. a T2W images of intracranial tumor in coronal views scanned using 7.0 T MRI on days 12, 25 and 39 after U251-GSCs injection. b The intracranial tumor growth curve of the shLGR5 (n = 5), Wnt-C59 (n = 5) and shCtrl (n = 5) U251-GSCs. Error bars represent the mean ± SD (n = 5, two-way ANOVA). c Overall survival time (OS) of xenograft mice in the shLGR5 (n = 5), Wnt-C59 (n = 5) and shCtrl (n = 5) U251-GSCs analyzed by Log-rank test. d IHC staining and quantitative analyses of LGR5, Ki67, Active-β-catenin, SOX2 and CD44 (magnification × 200). e Spearman correlation analysis of LGR5 and Active-β-catenin between shLGR5 and shCtrl U251-GSCs (P < 0.001, r = 0.8940, n = 10). f Spearman correlation analysis of LGR5 and Ki67 between shLGR5 and shCtrl U251-GSCs (P < 0.01, r = 0.7803, n = 10). g Spearman correlation analysis of LGR5 and SOX2 between shLGR5 and shCtrl U251-GSCs (P < 0.01, r = 0.8182, n = 10). h Spearman correlation analysis of LGR5 and CD44 between shLGR5 and shCtrl U251-GSCs (P < 0.01, r = 0.8788, n = 10). i T2W images of intracranial tumor in coronal views scanned using 7.0 T MRI on days 10, 20 and 36 after 8591-GSCs injection. j The intracranial tumor growth curve of the shLGR5 (n = 5), Wnt-C59 (n = 5) and shCtrl (n = 5) 8591-GSCs. Error bars represent the mean ± SD (n = 5, two-way ANOVA). k Overall survival time (OS) of xenograft mice in the shLGR5 (n = 5), Wnt-C59 (n = 5) and shCtrl (n = 5) 8591-GSCs analyzed by Log-rank test. l IHC staining and quantitative analyses of LGR5, Ki67, Active-β-catenin, SOX2 and CD44 (magnification × 200). m Spearman correlation analysis of LGR5 and Active-β-catenin between shLGR5 and 8591-GSCs (P < 0.01, r = 0.8343, n = 10). n Spearman correlation analysis of LGR5 and Ki67 between shLGR5 and shCtrl 8591-GSCs (P < 0.05, r = 0.7372, n = 10). o Spearman correlation analysis of LGR5 and SOX2 between shLGR5 and shCtrl 8591-GSCs (P < 0.01, r = 0.8545, n = 10). p Spearman correlation analysis of LGR5 and CD44 between shLGR5 and shCtrl 8591-GSCs (P < 0.05, r = 0.7333, n = 10). ***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, not significant