Fig. 4

Blocking IL-6 signaling with IL-6 neutralizing antibody or IL-6 receptor antibody abrogates the DCIS.com migration and proliferation enhanced by co-culture with human preadipocytes. Experimental scheme illustrating the transwell culture and 3D culture of DCIS.com (DCIS.com) and human preadipocytes (hPreAd). a Proliferation and migration analysis of DCIS.com treated with IL-6 (50 ng/ml), as assessed by MTT assay of six independent experiments. b Proliferation and migration analysis of DCIS.com in the presence or absence of IL-6 neutralizing antibody (IL-6 NAb, 3 μg/ml) or IL-6 receptor antibody (IL-6Rα NAb, 3 μg/ml), as assessed by crystal violet assay of three independent experiments. c Proliferation and migration analysis of DCIS.com in co-culture in the presence or absence of IL-6 neutralizing antibody (IL-6 NAb, 3 μg/ml) or IL-6 receptor antibody (IL-6Rα NAb, 3 μg/ml), as assessed by crystal violet assay of six independent experiments. d Proliferation analysis of hPreAd in the presence or absence of IL-6 neutralizing antibody (IL-6 NAb, 3 μg/ml) or IL-6 receptor antibody (IL-6Rα NAb, 3 μg/ml), as assessed by crystal violet assay of six independent experiments. e Fluorescence microscopy images of DCIS.com spheroids in 3D Matrigel culture in the presence or absence of with IL-6 (50 ng/ml), IL-6 NAb (3 μg/ml) or IL-6R NAb (3 μg/ml). All data are presented as the means ± standard errors