Fig. 4

PDX serial transplants. a Representative images of PDXs serially transplanted. F1 and F3 transplant generations were evaluated 6 and 12 months after first injection in mice, respectively. b Histogram showing the percentage of engraftment from F0 until F3 for three G3 and three G4 tumors when orthotopically injected in NSG mice. Ten mice for each tumor (three G3 and three G4) have been injected for each transplant. In F0 and F1 all tumors engrafted in all mice. In F2 and in F3 all tumors (same situation in G3 and G4 tumors) engrafted but the percentage represents number of mice which generated tumor xenograft-masses calculated over total injected. c Schematic representation of the lentiviral vector TW-LUC-EGFP. d Representative images of Luciferase expression of ccRCC population (F0) injected mice acquired by IVIS imaging system at 15, 50, and 90 days after first injection. e Luciferase Photon emission analysis of xenograft tumors at 15, 50, 90 day during F1 and F3 transplant generations, respectively, by IVIS imaging system. For the experiments five mice per G3 and G4 tumors were used. Data were reported as mean and Standard Deviation (f) Representative Haematoxylin and Eosin staining of two PDXs at F2 and F5 transplant generations were reported. F5 represent 18 months after first injection. Microscope Nikon Eclipse 55i, magnification 20×. Each F represents murine recipient generation of 3 months (90 days). F0 represents 3 months after first injection; F1 represents 3 months after second injection; F2 represents 3 months after third injection; F3 represents 3 months after fourth injection and so on