Fig. 2

AGAP2-AS1 promotes proliferation and suppresses apoptosis of breast cancer cells. a The oligonucleotides labeled with GFP green fluorescence were transfected as described in the methods. b Transfection efficiency was identified by detecting AGAP2-AS1 expression via RT-qPCR. c MTT assay showed the functional effect of AGAP2-AS1 on cell proliferation of breast cancer cells. d Colony formation assay was used to determine the functional role of AGAP2-AS1. e Immunofluorescence analysis of Ki-67 expression in breast cancer cells after infection with respective oligonucleotides. f FACS apoptosis analysis of cells with overexpression or knockdown of AGAP2-AS1. g, h Cell viability was evaluated in cells treated with trastuzumab (0.5 mg/mL) after infection of respective oligonucleotides. i TUNEL assay was used to determine the function of AGAP2-AS1 for trastuzumab-induced cell apoptosis. *p < 0.05, **p < 0.01, ***p < 0.001