Fig. 3

MyD88 is a downstream target of AGAP2-AS1 function in breast cancer. a RNA pull-down assay was performed to verify the enrichment of MyD88 by AGAP2-AS1 in the breast cancer cells. b Schematic diagram of the network of MyD88 based on ENCODE database (http://genome.ucsc.edu). c, d MyD88 was positively regulated by AGAP2-AS1 at both transcript and protein levels in SKBR-3 and BT474 cells. e MyD88 expression was determined in the trastuzumab-resistant and the parental breast cancer cells by western blot experiment. f MyD88 expression was detected via western blot after transfection of sh-MyD88 or Lv-MyD88. g Knockdown of MyD88 dramatically abrogated the effects of Lv-AGAP2-AS1 on cell proliferation whereas overexpression of MyD88 reversed the effect induced by sh-AGAP2-AS1. h Co-transfection of Lv-MyD88 reversed the effects of AGAP2-AS1 knockdown in SKBR-3/Tr cells whereas co-expression of sh-MyD88 abrogated the resistance induced by Lv-AGAP2-AS1 in the BT474 parental cells. *p < 0.05