Fig. 3

Endothelial cells induce autophagy by repressing AR. a Autophagosomes were detected by immunofluorescence for LC3-GFP in CWR22Rv1 cell in presence of HUVEC and/or DHT. b Western blot analysis of AR, p62, LC3II in presence of HUVEC and/or DHT. c Western blot analysis of AR, p62, LC3II in AR knockdown or parental cells in presence of HUVEC and/or DHT. d Autophagosomes were detected by immunofluorescence for LC3-GFP in CWR22Rv1 or AR knockdown CWR22Rv1 in presence of HUVEC and/or DHT. e Western blot analysis of AR, Atg5, Beclin-1, p62, LC3II. f C4–2 and CWR22Rv1 cells were cocultured with HUVECs in the presence or absence of CQ (Sigma) for 36 h, invasion assay was conducted