Fig. 2
From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
MIR17HG knockdown increases the permeability of glioma-conditioned normal BBB and decreases the expression of tight junction-related proteins. (a) Relative MIR17HG expression in ECs and GECs on quantitative real-time PCR. Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. the EC group. (b) Transfection efficiency of shMIR17HG. (c) Effects of MIR17HG knockdown on TEER values in the in vitro glioma-conditioned normal BBB model. (d) Effects of MIR17HG knockdown on HRP flux in the in vitro glioma-conditioned normal BBB model. (e) Effects of MIR17HG knockdown on the expression of tight junction-related proteins on western blot. Data of B-E represent the mean ± SD (n =3, each). *P < 0.05 vs. the shMIR17HG NC group, **P < 0.01 vs. the shMIR17HG NC group. (f) Effects of MIR17HG knockdown on the expression of tight junction-related proteins and their distributions on immunofluorescence (n = 3, each). Scale bar, 30 μm