Fig. 3
From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
piR-DQ590027 negatively regulates MIR17HG and MIR17HG is involved in piR-DQ590027-induced changes of the permeability of glioma-conditioned normal BBB and expressions of tight junction-related proteins. (a) Relative MIR17HG expression levels in piR-DQ590027-overexpressing or knockdown GECs on quantitative real-time PCR. Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. the piR-DQ590027 (+) NC group; #P < 0.05 vs. the piR-DQ590027 (-) NC group. (b) Relative luciferase activity in HEK293T cells co-transfected with pmirGLO-MIR17HG-Wt (pmirGLO-MIR17HG-Mut) and agomir-piR-DQ590027. Data represent the mean ± SD (n = 3, each). *P < 0.05 vs. the MIR17HG-Mut+ piR-DQ590027 (+) group. (c) TEER value was measured to evaluate the effects of MIR17HG and piR-DQ590027 on glioma-conditioned normal BBB integrity. (d) HRP flux was measured to evaluate the effects of MIR17HG and piR-DQ590027 on the permeability of glioma-conditioned normal BBB. (e) Western blot was performed to evaluate the effects of MIR17HG and piR-DQ590027 on the expression of tight junction-related proteins. Data of c-e represent the mean ± SD (n = 3, each). **P < 0.01 vs. the shMIR17HG-NC+piR-DQ590027 (+) NC group