Table 1 Primers and probes used for RT-qPCR
From: Mechanism of piR-DQ590027/MIR17HG regulating the permeability of glioma conditioned normal BBB
Primer or Probe | Gene | Sequence (5′- > 3′) or Assay ID |
|---|---|---|
Primer | MIR17HG | F:TCAGGAGTTCGAGACCAACC |
R:TGCCTCAGCCTCCAGAGTAG | ||
FOXR2 | F:CTGTGAACCCAATCTGTGGA | |
R:GGCTGAGGGAAAGGAGAAAT | ||
GAPDH | F: ACAGTCAGCCGCATCTTCTT | |
R: GCCCAATACGACCAAATCC | ||
Probe | MiR-153 | rs180704631 |
MiR-377 | rs774685804 | |
U6 | 001973(Applied biosystems) |