Fig. 2

Effect of CA on mitochondria dependent apoptosis in human prostate cancer cells. a Mitochondrial membrane potentials (ΔΨm) were assessed with JC-1 staining by flow cytometry. b The expression of cell key apoptosis-related proteins was detected by Western-blotting and they were statistically analyzed (c). The PC-3 and DU145 cells was pre-treated with caspase inhibitor (10 μM Z-VAD-FMK) for 3 h and followed by incubation with or without CA for 24 h, respectively. d The cell viability was determined by MTT assay. The results are presented as mean ± SD and described as column chart *p < 0.05 and **p < 0.01 as compared with corresponding group. e Effect of CA induced-Cytochrome c release was analyzed by immunofluorescence staining