Fig. 4

IRE-1-mediated ASK1-JNK activation plays a key role in CA-induced cell death. aThe PC-3 and DU145 cells were treated, respectively, by CA for 24 h at doses of 5, 10, 15 μM. The p-IRE-1, IRE-1, p-ASK1 and ASK1 protein expression were measured by Western blotting, and the bands were scanned and data of statistically analysis were listed in (b). Data represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 as compared with untreated control. The cells transfected with IRE-1 siRNA or control siRNA were treated with CA (10 μM) for 24 h. The protein expression was measured by western blotting (c), Densitometric analysis of protein bands was showed in (d). eThe cell viability was determined by MTT assay. The results are presented as mean ± SD and represent three individual experiments. *p < 0.05 and **p < 0.01 as compared with corresponding group