Fig. 6

Downregulation of AKT phosphorylation seems to be a secondary event associated with the PERK/CHOP/TRIB3 pathway activation. Gene and protein levels of TRIB3 in the PC-3 and DU145 cells were detected by RT-PCR (a) and western blot (b) after treatment with (5, 10, 15 μM). The cells were transfected with CHOP siRNA or control siRNA, respectively and treated with CA for 24 h. c The protein expression of CHOP, TRIB3 and p-AKT was measured by western blotting. d Protein levels were quantified by grayscale scan and the GAPDH was used as the loading control. The results are presented as mean ± SD and represent three individual experiments. *p < 0.05 and **p < 0.01 as compared with corresponding group. eThe cell viability was determined by MTT assay. The results are presented as mean ± SD. *p < 0.05 and **p < 0.01 as compared with corresponding group. f Overexpression of CHOP in PC-3 and DU145 cells that were treated with CA for 24 h. CHOP, AKT, p-AKT and TRIB3 protein expressions were determined by western blot. g Protein levels were quantified by grayscale scan and the GAPDH was used as the loading control. h MTT assay demonstrated that CHOP overexpression aggravated CA-induced inhibition of cell viability in PC-3 and DU145. The results are presented as mean ± SD and represent three individual experiments. *p < 0.05 and **p < 0.01 as compared with corresponding group