Fig. 4

PHF8 is involved in transcriptional activation of FIP200, SNAI1 and VIM. a Effect of PHF8-silencing on mRNA expression of key autophagy-related genes. b The relationship between PHF8 expression and FIP200 expression by Gene Expression Profiling Interactive Analysis (GEPIA, http://gepia.cancer-pku.cn/). c, d Western-blot analysis of FIP200 expression in SMMC-7721 and Huh7 cells with PHF8 knockdown and HepG2 and SK-Hep-1 cells with PHF8 exogenous overexpression. e Schematic illustration of the sites of primer amplicons on promoter regions of FIP200, VIM, SNAI1, CDH1 and CDH2 for ChIP analysis. f, g Combining ChIP and qRT-PCR analysis to measure the levels of PHF8 presence at promoter of FIP200, VIM, SNAI1, CDH1 and CDH2 in SMMC-7721 and Huh7 cells with PHF8 knockdown. * P < 0.05, ** P < 0.01, NS, no significance. Data were presented by mean ± SD from three independent experiments