Fig. 4

E2F5 and PFTK1 are involved to promote PCa cell proliferation and cell cycle progression in vitro. LnCap and 22RV1 cells were transfected with siRNAs of E2F5 and PFTK-1 respectively for 72 h; siControl served as negative control. a Representative photographs of colony formation assay and (b) quantification of the cell colonies formation were used to determine the proliferative ability of PCa Cells. c and d MTS assays revealed cell growth curves of indicated cells in every 24 h. e and f Flow cytometric determination of proportion of indicated cells in distinct cell cycle phases. g-j RT-qPCR and Western blot analysis the gene and protein expression of CDK2 and CDK4. LnCap cells were infected by lenti-E2F5 and PFTK1 to overexpress E2F5/PFTK1. Lenti-vector served as negative control. k and l The cell colony formation was used to determine the proliferative ability. m MTS assays revealed cell growth curves in every 24 h. n Western blot analysis the expression of CDK2 and CDK4 indicated cells. The results were plotted as the mean ± SEM of three independent experiments, with at least three replicates in each independent experiment. (*P < 0.05, **P < 0.01; #P < 0.05, ##P < 0.01)