Fig. 5

E2F5 and PFTK1 are a functional targets of miR-1-3p suppression of PCa cells proliferation and cell cycle progression. LnCap cells were co-transfected with miR-1-3p inhibitor and siRNA of E2F5 and PFTK-1 respectively for 72 h, inhibitor-NC and siControl served as respective negative control. a The effect of concomitant knockdown of miR-1-3p and E2F5 or PFTK1 on LnCap cells growth rates as measured by MTS assays. b and c Western blot analysis of E2F5 and PFTK1 protein levels in indicated cells. GAPDH was used as the loading control. d The effect of concomitant knockdown of miR-1-3p and E2F5 or PFTK1 on LnCap cells proliferative ability as determined by colony formation assays. e-f The proportion of indicated cells in distinct cell cycle phases as identified by FACS. Results were plotted as the mean ± SEM of three independent experiments, with at least three replicates in each independent experiment. (*P < 0.05, **P < 0.01)