Fig. 4

SLCO4A1-AS1 interacts with β-catenin. a RNA pulldown using biotin-labeled probe or intron control and sample lysates, followed by SDS-PAGE electrophoresis, silver staining and MS identification. b RNA pulldown showed that SLCO4A1-AS1 interacted with β-catenin in HCT116 and SW480 cells. SLCO4A1-AS1 was labeled with biotin. c RNA IP showed that β-catenin enriched SLCO4A1-AS1 in HCT116 and SW480 cell lysates. d β-catenin enriched SLCO4A1-AS1 in CRC sample cell lysates. e SLCO4A1-AS1 co-localized with β-catenin in CRC sample cells as shown by RNA fluorescence in situ hybridization (RNA-FISH). The scale bar was 10 μm. f, g Domain mapping showed that SLCO4A1-AS1 (nt: 900~ 1200) interacted with β-catenin and was indispensible. d900~ 1200 represented deletion of nt 900~ 1200. h RNA electrophoretic mobility shift assay (RNA-EMSA) showed that biotin-labeled SLCO4A1-AS1 (nt: 900~ 1200) directly bond to β-catenin. *p < 0.05 and **p < 0.01