Fig. 3

US exposure induces P-gp down-regulation is dependent on intracellular ROS generation. a US induced ROS generation in MCF-7/ADR and HEPG2/ADM cells which could be blocked by pre-treated with 5 mM NAC for 2 h (scale bar = 50 μm); Representative images of DCFH-DA staining in MCF-7/ADR and HEPG2/ADM cells 24 h post-treatment; N = 3; data are represented as mean ± s.d; *P < 0.05 compared with CON group; (b-d) Pre-treated with 5 mM NAC for 2 h inhibited US-induced P-gp down-regulation; Q-PCR assays (b), Western blot assays (c-d); (e-j) MCF-7/ADR and HEPG2/ADM cells were pre-treated with or without 5 mM NAC for 2 h before exposure to US+ADM. ADM intracellular concentration was determined by measuring fluorescent intensity (e); Cell viability was determined by MTT assay (f); Percentage of proliferative cell was determined by EDU staining (g-h, scale bar = 50 μm); Percentage of cell apoptosis was determined by TUNEL staining (i-j, scale bar = 50 μm); N = 3; data are represented as mean ± s.d; *P < 0.05 compared with ADM group