Fig. 2
From: Inhibition of STAT3 blocks protein synthesis and tumor metastasis in osteosarcoma cells
NP induces apoptosis in osteosarcoma cells in a dose-dependent manner. a, 143B and MG63 cells were treated with vehicle (Veh) or indicated NP (3.0 μM) for 24 h, and apoptotic morphologic changes were evaluated by fluorescence microscopy using Hoechst 33342. b-d, 143B and MG63 osteosarcoma cells were treated with Veh or indicated concentrations of NP for 24 h and evaluated by flow cytometry with Annexin V-FITC and PI staining. The number of apoptotic, necrotic, and live cells was quantitated. e and f, 143B and MG63 cells were treated with NP at the indicated concentrations and time. The expressions of cleaved PARP, caspase-3 were determined by Western blot using specific antibodies. The data are representative of 3 independent experiments. *P < 0.05 versus vehicle control; ** P < 0.01 versus vehicle control