Fig. 5

Inhibition of NHE1 deceases angiogenesis by reducing TAMs and associated cytokines. a Regression correlation analysis of NHE1 mRNA expression and monocytes/macrophages metagene score in CGGA dataset. b TAMs related genes were enriched in the areas of microvascular proliferation and hyperplastic blood vessels from the Ivy Glioblastoma Atlas Project. c Infiltration of Iba1⁺ cell in the tumor borders and tumor cores of SB28-GFP glioma-bearing mice. T, tumor. Data are means ± SEM from four independent experiments. * p < 0.05. ***, p < 0.001. d Representative flow cytometric profile showing gating strategy of microglia (CD11b⁺/CD45^int) and macrophages (CD11b⁺/CD45^high) in the same protocol as described in Fig. 4a. Data are means ± SD from five independent experiments. ns, no significance; ***, p < 0.001. e Representative flow-cytometry plot of TAMs stained for P2RY12 in fluorescence minus one (FMO) control, Veh, and HOE. Data are means ± SD from five independent experiments. *, p < 0.05; ***, p < 0.001. f Inflammatory profile of TAMs stained for CD16/32 and CD206. Data are means ± SD from five independent experiments. **, p < 0.01. g Flow-cytometry plots are shown in Additional file 1: Figure S6. Bar graphs show data summary of cytokines for TAMs. Data are means ± SD from five independent experiments. *, p < 0.05. **, p < 0.01