Fig. 5

UCHL1 promotes RA-increased neural differentiation markers of NB cells. SH-SY5Y cells were treated with DMSO or RA (10 μM) for 3 and 5 days, and SK-N-BE (2) cells were treated with DMSO or RA (10 μM) for 7 days. a-b The protein expression of TH, GAP43 and UCHL1 were measured by immunoblotting analysis at the indicated times and the densitometry of the bands was quantified using ImageJ software. c-d The mRNA expression levels of TH, GAP43, TUBB3 and NPY were measured by quantitative real-time PCR at the indicated times. SH-SY5Y cells were treated with RA (10 μM) for 3 days in the presence of LDN57444 (10 μM) or not. e The protein expression of TH, GAP43 and UCHL1 were measured by immunoblotting analysis and the densitometry of the bands was quantified using ImageJ software. f The mRNA expression levels of TH, GAP43, TUBB3 and NPY were measured by quantitative real-time PCR. Values are shown as mean ± S.E.M. and statistical significance indicated as * P < 0.05