Fig. 6

HCQ induces the transition of M2-TAMs to M1-like macrophages. a The representative flow cytometric histogram and quantification of CD206 and MHC II in TAMs (F4/80⁺ TILs), which were isolated from PBS- or HCQ-treated Lewis-bearing mice (n = 3). b TAMs were sorted from PBS- or HCQ-treated Lewis-bearing mice (n = 3), and the mRNA expression of Nos2, Ifng, IL12b, IL1b, Arg1, Tgfb1, IL10 and Ido1 was analysed. c TAMs were sorted from Lewis-bearing mice and treated with HCQ or not in vitro. The mRNA expression of Nos2 and Arg1 was analysed (n = 3). d Quantification of CD80, CD86 and MHC II in TAMs with or without HCQ treatment in vitro (n = 3). e TAMs were treated with HCQ or not in vitro. The mRNA expression of Ifng, IL12b, IL1b, IL6, Tgfb1, IL10 and Ido1 was analysed (n = 3). f TAMs were treated with HCQ or not, and the supernatant was collected to culture CFSE-labelled CD8⁺ T cell with CD3/CD28 bead stimulation. After 72 h, the histogram of CFSE was analysed by flow cytometry (n = 3). For all graphs, the error bars indicate the mean ± s.e.m., *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference. The data shown are representative of three independent experiments