Fig. 1

HOTAIRM1 is an antisense transcript to the HOXA1 gene and up-regulates in glioma tissues and GBM cells. a Schematic representation of HOXA gene cluster on human chromosome 7p15.2. The RNA expression of HOTAIRM1 was analyzed using quantitative PT-PCR, b in normal brain and glioma (WHO I - IV) tissues; c in normal brain, low grade glioma (WHO I and II) and high grade glioma (WHO III and IV) tissues; d in HA cell, established and primary GBM cell lines; with the GAPDH gene as an internal control, and error bars represent the SEs of three independent experiments. **P < 0.01, *** P < 0.001. e RNA-FISH analysis of HOTAIM1 RNA in A172 and U87 cells, and GBM tissues, and photomicrograph with confocal laser microscopy (20×)